Whole-genome shotgun sequencing of mitochondria from ancient hair shafts.
نویسندگان
چکیده
Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections.
منابع مشابه
Comment on "Whole-genome shotgun sequencing of mitochondria from ancient hair shafts".
Gilbert et al. (Reports, 28 September 2007, p. 1927) reported that "hair shafts surpass comparably stored bone as an aDNA source [...] in regard to preservation and concentration of mtDNA." When experimental parameters are carefully controlled for, including adequate sampling, quantitative polymerase chain reaction analysis, and modeling the decay of DNA, the general importance of this claim is...
متن کاملResponse to Comment on “Whole-Genome Shotgun Sequencing of Mitochondria from Ancient Hair Shafts”
, 857b (2008); 322 Science et al. M. Thomas P. Gilbert, Shafts" Sequencing of Mitochondria from Ancient Hair Response to Comment on "Whole-Genome Shotgun www.sciencemag.org (this information is current as of November 12, 2008 ): The following resources related to this article are available online at http://www.sciencemag.org/cgi/content/full/322/5903/857b version of this article at: including h...
متن کاملSupporting Online Material for Whole-Genome Shotgun Sequencing of Mitochondria from Ancient Hair Shafts
M. Thomas P. Gilbert, Lynn P. Tomsho, Snjezana Rendulic, Michael Packard, Daniela I. Drautz, Andrei Sher, Alexei Tikhonov, Love Dalén, Tatyana Kuznetsova, Pavel Kosintsev, Paula F. Campos, Thomas Higham, Matthew J. Collins, Andrew S. Wilson, Fyodor Shidlovskiy, Bernard Buigues, Per G. P. Ericson, Mietje Germonpré, Anders Götherström, Paola Iacumin, Vladimir Nikolaev, Malgosia Nowak-Kemp, Eske W...
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The performance of hybridization capture combined with next-generation sequencing (NGS) has seen limited investigation with samples from hot and arid regions until now. We applied hybridization capture and shotgun sequencing to recover DNA sequences from bone specimens of ancient-domestic dromedary (Camelus dromedarius) and its extinct ancestor, the wild dromedary from Jordan, Syria, Turkey and...
متن کاملPulling out the 1%: whole-genome capture for the targeted enrichment of ancient DNA sequencing libraries.
Most ancient specimens contain very low levels of endogenous DNA, precluding the shotgun sequencing of many interesting samples because of cost. Ancient DNA (aDNA) libraries often contain <1% endogenous DNA, with the majority of sequencing capacity taken up by environmental DNA. Here we present a capture-based method for enriching the endogenous component of aDNA sequencing libraries. By using ...
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عنوان ژورنال:
- Science
دوره 317 5846 شماره
صفحات -
تاریخ انتشار 2007